Software for hla typing kits

Designed to address the diverse needs of hla labs, our molecular typing product family includes next generation sequencing ngs, sequencebased typing sbt, realtime pcr, sequencespecif ic primers ssp, and reverse sequencespecif ic oligonucleotide rsso reagents and accessories. Hla typing by nextgeneration sequencing ngs generates unambiguous, phaseresolved hla typing results using a single assay, system, and analysis program. Hla typing a comparison of serology and dna techniques. We offer high quality hla sbt typing kits and hla ssp typing kits to analyze the human leukocyte antigens for the tissue typing process.

Click in the status information panel of the screengel software to move the buffer tray holder to the front of the instrument. In addition to our reagent kits and software we offer tissue typing services to facilitate your hla research. Ideal solution for both low volume and high throughput laboratories. Userfriendly software included freeofcharge our own multifunction and userfriendly typing software. Our ssp product was introduced in 1992, setting the bench mark for hla. Learn how to analyze linkseq sabr hla typing kits using the newly released version 6. Our kits are designed for ease of use and yield high quality results.

Hla test kits used for matching of donors and recipients in transfusion and. Reagents officially adopted by japan marrow donor program wakflow hla typing kits have been used for jmdp donor registration since 2005. Secore sbt kits secore kits are high resolution hla typing products based on sequence based typing. Hla types were determined after analysis using biocalculator software and hla genotyping software. The applied biosystems 3500 dx3500xl dx cs2 genetic analyzers, invitrogen secore hla sequencing kits, and utype dx hla sequence analysis software constitute the first fda 510kcleared sequencebased system for hla typing in the united states.

In addition to the diagnostic secore hla assay that you can run in ivd mode, the 3500 dx genetic analyzers cs2 systems allow you to develop new assays in ruo mode. The sbtexcellerator hlag kit is a novel hlag assay that allows high level of sequencing coverage and genotyping resolution. Jul 11, 2019 jul 11, 2019 heraldkeepers hla typing for transplant market size was valued over usd 390. Longrange amplification kit that leverages the power of the holotype hla. Linkseq hla typing kits linkseq is the most complete solution for high throughput laboratories interested in fast and easy typing of all 11 loci. Europe hla sequencing based typing sbt kits loci a, b and c analysis sequences of. The human leukocyte antigen hla gene cluster plays a crucial role in adaptive immunity and is thus relevant in many biomedical applications. As the leader in hla for over 30 years, we develop and distribute a comprehensive line of hla typing and antibody detection products, along with instrumentation and software that are used to simplify and automate testing procedures and final. Jul 11, 2019 heraldkeepers hla typing for transplant market size was valued over usd 390. Ngs hla typing kits ngs chimerism typing kits scisco genetics. We implemented a high quality management system according to iso 9001, iso 485 and directive 9879ec for medical device manufacturer. Transplant diagnostics thermo fisher scientific us. This broad range of products provides both molecular and serological hla typing solutions. The systems have been validated using secore hla kits and utype dx software for powerful, rapid data analysis and high accuracy in hla typing.

Rather than adapting software used for sanger sequencing or other ngs applications like oncology, we spent more than seven years developing a solution that is uniquely suited for highresolution hla typing. Typing kits qtype is a real time pcr assay that utilizes the multiplexing capabilities of hydrolysis probes to increase the capacity for unique assays in each well. Hla typing the worlds first freezedried hla ssp delivers unique simplicity, accuracy and ease of use. Typing for hladpb1 needs only a few microbead populations, whereas hlab typing on an intermediate resolution level needs almost the complete set. Unambiguous, phaseresolved hla typing in a single assay using proven illumina ngs technology and assign analysis software. Using hydrolysis probes allows quick and robust typing from low input dna concentrations and removes the risk of misinterpreting primer dimers and other pcr artefacts seen in real. The trusight hla v2 sequencing panel provides clinical researchers with a broadcoverage, ultrahighresolution human leukocyte antigen hla typing solution for simple, rapid assessment of the hla region in a single assay. The software is designed to analyze data from libraries prepared with illumina trusight hla sequencing panels and then sequenced on an illumina sequencer. Reagent analysis software hla real time pcr typing kits nucleic acid extraction kits analysis software. Advanced hla genotyping software for genetics genomics researchers to interpret any ngs data.

Longrange amplification kit that leverages the power of the. Our diagnostic tests are used to determine donor compatibility pretransplant, and posttransplant to monitor hla antibodies that can lead to rejection. Shiva scientific company was founded in by senior immunologist dr. Trusight hla v2 sequencing panel hla typing with analysis. Hla typing a comparison of serology and dna techniques article pdf available in international journal of human genetics 41. The lifecodes hla typing kits rapid utilize sso methodology to identify hla alleles present in a locusspecific pcramplified sample. Secore hla sequencebased typing thermo fisher scientific us.

Mcd is the uk, ireland and australian distributor for bag healthcare transplantation diagnostics products. The software that powers the mia fora ngs flex analysis was developed based on immucors extensive experience with hla typing. In most laboratories, conventional typing method was performed by cell cytotoxicity tests or fluorescence serology with specific antibodies, and now dna typing method using pcr is widely being used due to its specificity. Hlahuman leukocyte antigen is major gentietic marker for transplantation and specific disease. Advanced hla genotyping software for genetics genomics researchers to. Hla typing using olerup ssp kits and the qiaxcel advanced system 052016 5 5. When an organ is from a deceased donor, hla testing is performed to match it as quickly as possible to a potential recipient or recipients.

We process various samples such as whole bloodcord blood, nucleated cells, plasma, packed rbc, buccal swab, genomic dna and so on. For analysis and typing of pcr products from different hla loci using the qiaxcel advanced and the helmbergscore software. While nextgeneration sequencing data are often available for a patient, deducing the hla genotype is difficult because of substantial sequence similarity within the cluster and exceptionally high variability of the loci. For typing, the ssp method uses allelespecific primers in the amplification reaction. Hla typing assay by ngs for use on illumina sequencers combined with hla twin software. This product provides intermediate resolution typing for hla a, b, c, drb1, drb345, dqa1, dqb1, dpa1, and dpb1, as well as common null alleles. The bd hlab27 system is a qualitative twocolor direct immuno. Is there any software to analyze hlatyping a,b,dr data for. Our ngs solution for hla class i and ii typing is carried out via multiplex pcr of hla specific amplicons. Home products hla tissue typing hla ssp typing kits. International group sincerely welcomes business partnerships. Secore kits are invitrogens latest line of high resolution hla typing products based on sequenced based typing the gold standard of allele identification.

Hla fusion has two software versions to support hla screening and dna typing products in either clinical diagnostics or research sciences. Ngs hla typing kits ngs chimerism typing kits scisco. Recommendations for premarket notification 510k submissions for nucleic acidbased human leukocyte. Typing kits hla sbt typing kits reagents and accessories analysis software. Enables high precision human leukocyte antigen hla typing even.

The olerup sbt product line includes a wide variety of typing kits for high. Dualalgorithm hla genotyping software for ngs data that delivers the most accurate hla genotyping. Hla typing using olerup ssp kits and the qiaxcel advanced. Immucor launches mia fora ngs flex hla typing assay. An appropriate strategy for class i molecular typing for unos laboratories should at least include generic level typing for all hla a and b types, typing for bw4 and bw6 epitopes, and typing for allelic subgroups of b15 and b40 that can distinguish the common alleles defining the serological splits. Morgan hla abc ssp typing kit catalog no33211 33210 kit size. Holotype hla ngs reagent analysis software hla real time pcr typing kits nucleic acid. With excellent exon coverage we can solve practically any ambiguity, allelic ambiguities by sequencing additional exon regions and genotypic ambiguities with group specific sequencing primers gssp. Interpretation of hla typing results for entry into unet optn. The products are used by trained professionals in medical settings for the purpose of determining hla phenotype. Life technologies receives fda 510k clearance for diagnostic use of sanger sequencing platform and hla typing kits lifes applied biosystems3500 dx genetic analyzer and secore kits. Allow the buffer tray holder to reach its stop position. Olerup ssp hla typing kits are qualitative in vitro diagnostic kits for the dna typing of hla class i and hla class ii alleles.

Linkseq is the most complete solution for high throughput laboratories interested in fast and easy typing of all 11 loci. Safe and rapid hla typing using the qiaxcel system qiagen. Immucor launches mia fora ngs flex hla typing assay immucor. In my lab i use olerupssp hlatyping tray kit for hlaa,b,dr typing of patient and donor prior to check the matchability for organ transplant.

Polysolver uses relatively low coverage whole exome sequencing wes data and a subsequent mutation detection pipeline that is based on the inferred alleles to detect mutations in hla genes. Phased 3field hla typing in a single assay using proven scisco genetics. Upadhyay with a vision to provide innovative solutions and technical support in the field of life sciences with core competence in transplant immunology. Antigen hla test kits used for matching of donors and recipients in. Hla typing using olerup ssp kits and the qiaxcel advanced system. First commercially available sbt hlag typing kit sbtexcellerator hlag brings fast and easy high resolution hlag sequencingbased typing sbt within reach. In addition we have real time pcr, conventional pcr, gel doc and gel electrphoresis. Dna sequencingbased typing became the gold standard for high resolution hla typing with the development of fluorescentdye labeled sequencing of pcr amplicons using automated genetic analysis equipment coupled with hla software analysis products. Mar 02, 2019 learn how to analyze linkseq sabr hla typing kits using the newly released version 6. Hla genotyping and antibody characterization using the. Sample to sequencing in gene kits upgrade to the power of the mia fora 3. Hla typing is also performed on unrelated individuals who wish to become a bone marrow donor through the donation registry. Morgan hla abdr ssp typing kit catalog no33111 33116 kit size. Hla typing sanger sbt kits shiva scientific company.

Hla typing on the 3500 dx offers labs an optimized, streamlined workflow with high resolution. While nextgeneration sequencing data are often available for a patient, deducing the hla genotype is difficult because of. Typing is performed with tbg morgan hla ssp typing kits for lowresolution hlatyping and tbg hlassure sbt kits for highresolution hlatyping. Hla typing kits mcd is the uk, ireland and australian distributor for bag healthcare transplantation diagnostics products. Exon and flanking intron sequences from the hla genes are amplified in 4 reactions, simultaneously tagged with index sequences to distinguish samples, pooled, and sequenced. Interpretation of hla typing results for entry into unet. The custom lab service results can be used for research use only. Achieving highresolution human leukocyte antigen hla typing results with conventional methods requires multiple assays, systems, and analysis programs. Sanger sequencing system and hla typing kits, 9 cap today.